What is the principle of micellar electrokinetic capillary chromatography?
In micellar electrokinetic capillary chromatography, surfactants are added to the buffer solution in concentration above their critical micellar concentrations, consequently micelles are formed; micelles that undergo electrophoretic migration like any other charged particle.
What is micellar capillary electrophoresis?
Micellar electrokinetic chromatography is a modified version of capillary electrophoresis which is used to separate a wide range of molecules, from inorganic ions to large biopolymers.
Is capillary electrophoresis chromatography?
Capillary electrochromatography (CEC) is a chromatographic technique in which the mobile phase is driven through the chromatographic bed by electroosmosis. High-performance liquid chromatography separates analytes by passing them, under high pressure, through a column filled with stationary phase.
What is the difference between electrophoresis and electroosmosis?
In electrophoresis, solid particles (macromolecules like nucleic acids or proteins) are moved using an electric field. But in electro-osmosis a liquid is moving. In electrophoresis, the support solid material is a gel. But it electro-osmosis it can be a gel, membrane, capillary, etc.
What is meant by electro-osmosis?
Electroosmosis is the flow of liquid that is in contact with a charged solid surface when an electric field is applied, and it becomes an important consideration with the increased surface area-to-volume ratio associated with small diameter capillaries.
What does MEKC stand for?
Micellar electrokinetic chromatography ( MEKC) is a chromatography technique used in analytical chemistry. It is a modification of capillary electrophoresis (CE), extending its functionality to neutral analytes, where the samples are separated by differential partitioning between micelles…
How do analytes move during MEKC separation?
During a MEKC separation, analytes distribute themselves between the hydrophobic interior of the micelle and hydrophilic buffer solution as shown in figure 1. . Analytes that solubilize completely within the micelles (analytes that are highly hydrophobic) should migrate at the micelle velocity,
How can I extend the elution range in MEKC?
In MEKC, however, techniques must be used to extend the elution range to separate strongly retained analytes. Elution ranges can be extended by several techniques including the use of organic modifiers, cyclodextrins, and mixed micelle systems.
What is the best surfactant for MEKC?
Sodium dodecyl sulfate (SDS) is the most commonly used surfactant in MEKC applications. The anionic character of the sulfate groups of SDS cause the surfactant and micelles to have electrophoretic mobility that is counter to the direction of the strong electroosmotic flow.
