What is SuperScript III?
Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) that was created by introduction of several mutations for reduced RNase H activity, increased half-life, and improved thermal stability.
What is SuperScript RT?
SuperScript II Reverse Transcriptase (RT) is a DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or from an RNA:DNA hybrid. This enzyme is genetically engineered by the introduction of point mutations in the RNase H active center to reduce RNase H activity.
What is SuperScript IV?
SuperScript® IV Reverse Transcriptase (RT) is a proprietary MMLV mutant with superior robustness and reliability in RT reactions. SuperScript® IV RT is the top choice for all RT-PCR and qRT-PCR applications in the SuperScript® product family.
What is RT protocol?
Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs. The following experiments can be used as basic RT protocols that can be modified to suit particular requirements.
What is cDNA in biology?
Complementary DNA (cDNA) is a DNA copy of a messenger RNA (mRNA) molecule produced by reverse transcriptase, a DNA polymerase that can use either DNA or RNA as a template. From: Encyclopedia of Genetics, 2001.
Where are reverse transcriptase found?
Reverse transcriptase is an enzyme found in retroviruses that converts the RNA genome carried in the retrovirus particle into double-stranded DNA. Reverse transcriptase first transcribes a complementary strand of DNA to make an RNA:DNA hybrid.
Where does reverse transcriptase come from?
reverse transcriptase, also called RNA-directed DNA polymerase, an enzyme encoded from the genetic material of retroviruses that catalyzes the transcription of retrovirus RNA (ribonucleic acid) into DNA (deoxyribonucleic acid).
What is supersuperscript III reverse transcriptase used for?
SuperScript® III Reverse Transcriptase is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability. The enzyme is used to synthesize cDNA at a temperature range of 42–55°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse…
Is superscript™ III RT Rnase-free?
SuperScript® III RT is RNase-free. SuperScript™ III Reverse Transcriptase (2,000 units total, at 200U/µL) is supplied with a vial (1 mL) of 5X first-strand buffer [250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM MgCl 2 ], and a vial (500 µL) of 100 mM DTT. Store at -20°C. Convenient, on-site access to the products you need.
What is Invitrogen superscript III?
Description Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) that was created by introduction of several mutations for reduced RNase H activity, increased half-life, and improved thermal stability.
How is superscript used to synthesize cDNA?
The enzyme can be used to synthesize cDNA at a temperature range of 45–55°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. Because SuperScript® III RT is not significantly inhibited by ribosomal and transfer RNA, it can be used to synthesize cDNA from total RNA.
