What is the meaning of HPTLC?
High-performance thin-layer chromatography (HPTLC) is an enhanced form of thin-layer chromatography (TLC). A number of enhancements can be made to the basic method of thin-layer chromatography to automate the different steps, to increase the resolution achieved, and to allow more accurate quantitative measurements.
How does high performance TLC work?
High Performance Thin Layer Chromatography (HPTLC) gives much greater resolution and separation of components than normal TLC. It uses chromatographic stationary phases with excellent separation efficiency and employs state of the art instrumentation for all steps in the procedure.
What is difference between TLC and HPLC?
HPLC software is capable of reporting precise and accurate results based on area counts of peaks. TLC quantifications are based on visual comparisons or spot intensity matching techniques which can be less quantitative in practice.
Why is high performance thin chromatography superior to thin layer chromatography?
HPTLC or high performance thin layer chromatography is the advanced form of thin layer chromatography (TLC). The analysis is faster than the TLC technique. The sensitivity of this method is also high. In HPTLC, several different samples can be separated in the same plate without any sample preparation step.
What is the clinical uses for thin layer chromatography?
PRINCIPLE. Thin layer chromatography (TLC) is used in specialty areas of the clinical laboratory. Tests include 1) screening for drug overdoses, 2) screening for aminoacidurias, 3) determination of L/S ratios, and 4) screening for galactosuria or fructosuria.
What is the difference between TLC and GC?
GC is a fully quantitative technique with software capable of providing accurate data such as peak area counts for quantitative estimations. The TLC technique requires subsequent treatment of the spots such as dissolving in suitable solvent or extraction for subsequent spectrophotometric determinations.
