What is the function of LARP1?
LARP1 binds to and regulates the translation of terminal oligopyrimidine motif (TOP mRNAs) and can directly interact with the 5′ cap of mRNAs. It has also been shown to interact with the 3′ end and coding regions (CDS) of other genes.
What is the function of LARP1 and where does it bind?
We show that LARP1 directly binds the cap and adjacent 5’TOP motif of TOP mRNAs, effectively impeding access of eIF4E to the cap and preventing eIF4F assembly. Thus, LARP1 is a specialized TOP mRNA cap-binding protein that controls ribosome biogenesis.
What is top mRNA?
TOP mRNAs are vertebrate transcripts which contain a 5’terminal oligopyrimidine tract (5’TOP), encode for ribosomal proteins and elongation factors 1alpha and 2, and are candidates for growth-dependent translational control mediated through their 5’TOP.
What does eIF4E bind to?
eIF4E is the cap-binding protein which, in synergy with proteins such as the helicase eIF4A and the scaffolding protein eIF4G, binds to mRNA, allowing the recruitment of ribosomes and translation initiation.
What binds with the 5 cap of mRNA?
The heterodimeric nuclear cap-binding complex (CBC) binds to the mono-methylated 5′ cap of eukaryotic RNA polymerase II transcripts such as mRNA and U snRNA. The binding is important for nuclear maturation of mRNAs and possibly in the first round of translation and nonsense-mediated decay.
What is LARP in biology?
The key to a good classroom LARP is creating situations where students must make decisions by applying their biological knowledge. …
Does LARP1 prevent translation?
LARP1 regulates translation and stability of this class of mRNAs through specialized 5’TOP motif (Fonseca et al., 2015; Lahr et al., 2015; Aoki et al., 2013) and cap recognition.
What is eif4?
eIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of the eIF4F pre-initiation complex. Almost all cellular mRNA require eIF4E in order to be translated into protein.
Is mRNA safe?
mRNA vaccines are safe and effective.
What is the function of eEF2?
Molecular Mechanisms of Memory Eukaryotic elongation factor 2 (eEF2) is a GTP-binding protein that mediates the translocation of peptidyl-tRNA from the A site to the P site on the ribosome (Moldave, 1985).
