How much GelRed do I add?
A 0.5 mL vial of GelRed® or GelGreen® is can be used to prepare 100 minigels (50 mL each) using the precast protocol, or for post-electrophoresis staining of 33 minigels in 50 mL staining solution per gel. Post-staining solution also can be re-used for staining two or more gels.
How do you dilute GelRed?
Post staining with GelRed™ is simple, requiring no destaining and no special buffer. Simply dilute the concentrated dye in 0.1 M NaCl or water and incubate the gel in the diluted dye solution for 30 minutes.
What is the purpose of GelRed?
GelRed is a sensitive, stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels.
Is GelRed a loading dye?
GelRed® Prestain Plus 6X DNA Loading Dye contains density agents, tracking dyes, and GelRed® dye. The 6X prestain loading dye is added to samples in place of gel loading buffer, and eliminates the need to add fluorescent DNA dye to the agarose gel during casting or after electrophoresis.
Is GelRed a carcinogen?
GelRedTM is a highly sensitive fluorescent nucleic acid dye that works when excited by a transilluminator. It was designed to replace EtBr, which is considered to be carcinogenic and mutagenic. GelRedTM is also known to be very sensitive without requiring a destaining step.
Is GelRed safer than ethidium bromide?
GelRed™ is a commercial DNA stain manufactured by Biotium. It is marketed as being the most safe, sensitive and robust nucleic acid gel stain- less mutagenic than ethidium bromide, but more stable in storage than SYBR®Safe. Like ethidium bromide, GelRed™ is visualized using UV light.
Is GelRed cheaper than ethidium bromide?
GelRed is more expensive than ethidium bromide but something to consider when doing a cost comparison is some labs may be able to save on hazardous waste disposal costs (as well as the time and hassle of decontaminating electrophoresis buffers) by switching from EtBr to GelRed.
Is GelRed light sensitive?
Protect from light. GelRed™ is a sensitive, stable and relatively safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EB) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. GelRed™ is far more sensitive than EB without requiring a destaining step.
Why is GelRed better than ethidium bromide?
GelRed® is much more sensitive than EtBr, and unlike SYBR® Gold, GelRed® can also be used as a highly sensitive precast gel stain. Another major advantage of GelRed® and GelGreen® DNA stain is their remarkable stability. You can store and handle the two nucleic acid staining dyes the same way you do with EtBr.
What is the difference between EB and GelRed®?
GelRed® is far more sensitive than EB without requiring a destaining step. GelRed® and EB have virtually the same spectra, so you can directly replace EB with GelRed® without changing your existing imaging system. GelRed® can be used to stain dsDNA, ssDNA or RNA in agarose gels via either precast or post gel staining.
What is the difference between GelRed® in water and in DMSO?
Biotium also offers GelRed® 10,000X in water (catalog no. 41003) and GelRed® 10,000X in DMSO (catalog no. 41002). GelRed® in water is a newer, safer formulation and our recommended format. We continue to offer GelRed® in DMSO for established users who do not wish to alter their protocols.
What is the use of GelRed stain?
GelRed® can be used to stain dsDNA, ssDNA or RNA in agarose gels via either precast or post gel staining. GelRed® can also be used to stain dsDNA, ssDNA or RNA in polyacrylamide gels via post gel staining. GelRed® is also compatible with downstream DNA manipulations such as restriction digest, sequencing, and cloning.
How do gelgelred™ and GelGreen™ work?
GelRed™ and GelGreen™ use a novel yet very simple concept to reduce genotoxicity by preventing the dyes from entering living cells. A DNA-binding dye can be made nonmutagenic or substantially less mutagenic by denying contact with genomic DNA in living cells.
