How do you ProLong gold Antifade reagent?
It comes ready-to-use (room temperatures storage and no mixing required)—just apply a drop to the sample, add a coverslip, cure, and image. ProLong Gold Antifade Mountant is not recommended for mounting samples containing fluorescent proteins, such as GFP.
How to use ProLong Gold Antifade Reagent with DAPI?
Product Usage Information Following immunostaining, cover samples with ProLong® Gold Antifade Reagent with DAPI #8961 and coverslip. To prevent fading, allow slides to cure in the dark, at room temperature for 24 hours, then seal the coverslips with clear lacquer, such as nail polish. Proceed with imaging.
How does ProLong gold work?
ProLong Gold mountant contains chemical components designed to protect fluorescent dyes from fading (photobleaching) during fluorescence microscopy experiments, and delivers this protection without significantly quenching the initial fluorescence signal.
How long does ProLong gold take to set?
24 hours
Like the original ProLong® antifade reagent, ProLong® Gold reagent will cure within 24 hours, and the sample can be saved for months after mounting.
How long does ProLong gold last?
[1] The product may also be stored at ≤–20°C. When stored as directed, the product is stable for at least 6 months.
How long does ProLong gold take to cure?
ProLong® Gold reagent causes little or no quenching of the fluorescent signal (Figure 1). Like the original ProLong® antifade reagent, ProLong® Gold reagent will cure within 24 hours, and the sample can be saved for months after mounting.
What is Antifade?
Filters. Referring to something that resists the dimming (fading) of color. The chemical additives in the anti-fade paint meant the color stayed vibrant year after year.
How long does it take ProLong gold to dry?
For samples mounted using the ProLong™ Gold or Diamond Antifade Mountant, allow the preparation to cure on a flat surface in the dark. Curing time may vary from a couple of hours to overnight, depending on the thickness of the sample and the relative humidity of the surrounding air. We recommend 24 hours curing time.
Does ProLong gold have glycerol?
When comparing this product to the competition’s mounting media, keep in mind that ProLong® Gold is a glycerol-based hard mount media. This means that it cures over 24 hours to a solid state so coverslips do not need to be sealed to the slide with products like nail polish or beeswax.
How long does vectashield last?
Curing formulations with choice of counterstain (DAPI) or no counterstain. 12 month product expiration date.
What is prolong® gold antifade mountant?
ProLong® Gold Antifade Mountant is a liquid mountant applied directly to fluorescently labeled cell or tissue samples on microscope slides. It contains chemical components designed to protect fluorescent dyes from fading (photobleaching) during fluorescence microscopy experiments, and delivers this protection without significantly…
What is the difference between DAPI nuclear stain and prolong® gold?
ProLong® Gold mountant is available with or without DAPI nuclear stain. ProLong® Gold Antifade Mountant is not recommended for mounting samples containing fluorescent proteins, such as GFP. For superior antifade protection of fluorescent proteins and fluorescent dyes, ProLong® Diamond Antifade Mountant is recommended.
What is the best antifade reagent for photobleaching?
ProLong ® Gold Antifade Reagent with DAPI offers enhanced resistance to photobleaching, is premixed and ready to use, and causes little or no quenching of the fluorescent signal. The reagent cures within 24 hours and samples can be saved for months after mounting.
Why is DAPI added to the mounting reagent?
The inclusion of DAPI within this mounting reagent eliminates the need for a separate nuclear counterstaining step. 4′, 6-diamidino-2-phenylindole, dihydrochloride (DAPI) is a blue fluorescent DNA dye that targets double-stranded AT clusters in the DNA minor groove (1).
